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A photomultiplier steps the emitted chemiluminescence, and outcomes are decided by way of a calibration curve produced by a two-stage calibration in addition to a master curve (5-place calibration) offered via the reagent barcode.

Aside from Wrong-optimistic effects, heterophile antibodies could cause falsely low outcomes if they bind to your variable locations in the seize antibody, mimicking the antigen to become measured and stopping troponin from binding.[89] The mostly utilised technique for minimizing the impact of HAMA on commercial immunoassays could be the addition of non-immune mouse immunoglobulin (IgG).

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The principle from the cTnT measurement is surely an ELISA one particular-move sandwich assay using streptavidin know-how and electrochemiluminescence detection.[fifty four] In the first incubation step (immunological response), the cTnT from the sample reacts using a biotinylated mouse monoclonal anti-cTnT antibody as well as a monoclonal cTnT-precise antibody labeled with a ruthenium complicated to variety a sandwich elaborate.

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